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KMID : 0380020060210060456
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Korean Journal of Biotechnology and Bioengineering
2006 Volume.21 No. 6 p.456 ~ p.460
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Stabilization of Alcohol Oxidase under Electrostimulation; Sugars, Hydrogels and Surfactants Effect
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Kim Beom-Su
Lee Kang-Min
Biellmann J. F.
Kim Kyung-Suk
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Abstract
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We investigated the activity and stability of alcohol oxidase from Hansenula sp., Pichia pastoris, and Candida boidinii under the electric stimulation. The activity and stability of alcohol oxidase depended on electric output voltage, electric stimulation time. This inactivation of the enzyme under electric stimulation could be recovered by stabilizing additives such as sugars, surfactants and hydrogels. These alcohol oxidase was more stable in trehalose, Triton X-100, Brji solution and alcohol oxidase from Hansenula is more stable than that from P. pastoris, and C. boidinii. The stabilizing of enzymes against electric stimulation showed a great potential use of enzymes in biotechnology and medical engineering fields.
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KEYWORD
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Electrostimulation, alcohol oxidase, stability
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